Associations of chemical composition and sources of PM2.5 with lung function of severe asthmatic adults in a low air pollution environment of urban Nagasaki, Japan.

Previous studies have linked ambient PM2.5 to decreased pulmonary function, but the influence of specific chemical elements and emission sources on the severe asthmatic is not well understood. We examined the mass, chemical constituents, and sources of PM2.5 for short-term associations with the pulmonary function of adults with severe asthma in a low air pollution environment in urban Nagasaki, Japan. We recruited 35 asthmatic adults and obtained the daily record of morning peak expiratory flow (PEF) in spring 2014-2016. PM2.5 filters were extracted from an air quality monitoring station (178 days) and measured for 27 chemical elements. Source apportionment was performed using Positive Matrix Factorization (PMF).

We fitted generalized linear model with generalized estimating equation (GEE) method to estimate changes in PEF (from personal monthly maximum) and odds of severe respiratory deterioration (first ≥ 15% PEF reduction within a 1-week interval) associated with mass, constituents, and sources of PM2.5, with adjustment for temperature and relative humidity.

EDTA
GE4011-100G 100 g
EUR 40

EDTA
GE4011-1KG 1 kg
EUR 70

EDTA
GE4011-250G 250 g
EUR 45

EDTA
GE4011-500G 500 g
EUR 54

EDTA Solution
2103-100
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EDTA plasma
90-1009 100 ml
EUR 2028
Description: BNP and NT-proBNP free plasma EDTA

Trypsin (-EDTA)
abx098244-100ml 100 ml
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EDTA Na2
abx082384-500g 500 g
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0.5M EDTA
E9000-010 100 ml
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0.5M EDTA
E9000-050 500 ml
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100G EDTA
NAT1090 100G
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500G EDTA
NAT1092 500G
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TRYPSIN/EDTA 1X (0,25% TRYPSIN/2,21 MM EDTA)
25-053-CI 100 mL/pk
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Description: Media Catalog; Cell Culture Reagents

Rabbit Plasma (EDTA)
88R-1029E 500 ml
EUR 230
Description: Sterile Rabbit Plasma with EDTA

EDTA, Free acid
EB0107 500g
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100 ML TRYPSIN/EDTA 1X (0,05% TRYPSIN/0,53 MM EDTA)
25-052-CI 100 mL/pk
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Description: Media Catalog; Cell Culture Reagents

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-010 100ml
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Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-050 500ml
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Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-101 1L
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Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8150-050 500 ml
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Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8150-801 8L
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Rabbit Plasma, Sterile, EDTA
31156-1 500mL
EUR 224
Description: Whole blood is collected from young (8 - 12 weeks) healthy New Zealand White or Californian rabbits from both sexes and fasted prior to collection. The blood is mixed with 2.5% Sodium EDTA (25ml Sodium EDTA per 250ml of the total volume). The plasma is separated from the blood cellular components by centrifugation. The plasma is pooled, passed through a 0.22-micron filter, bottled and stored at or below -10 °C.

DMNP-EDTA(caged calcium)
50050 5MG
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Normal Feline Plasma (EDTA)
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CA014-010 100ml
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Trypsin-EDTA(1X), 0.25%
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Trypsin-EDTA(1X), 0.25%
CA014-100 10x100ml
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Trypsin-EDTA(10X), 0.5%
CA015-100 10x100ml
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Trypsin-EDTA(10X), 0.5%
CA015-200 20x100ml
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Trypsin-EDTA(1X), 0.05%
CA020-010 100ml
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Trypsin-EDTA(1X), 0.025%
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Trypsin-EDTA(1X), 0.025%
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Trypsin-EDTA(1X), 0.025%
CA021-200 20x100ml
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Trypsin-EDTA(10X), 0.05%
CA022-010 100ml
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EDTA, Disodium Salt, Dihydrate
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EDTA, Disodium Salt, Dihydrate
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GL1464-100G 100 g
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EDTA tripotassium salt dihydrate
GL1464-25G 25 g
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EDTA disodium salt dihydrate
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EDTA disodium salt dihydrate
GE3023-1KG 1 kg
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EDTA disodium salt dihydrate
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EDTA disodium salt dihydrate
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EDTA tetrasodium salt dihydrate
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EDTA tetrasodium salt dihydrate
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1L EDTA 0.5M Solution
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EDTA, disodium salt, dihydrate
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Rabbit Plasma Non-Sterile, EDTA
31141-1 500mL
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Description: Whole blood is collected from young (8 - 12 weeks) healthy New Zealand White or Californian rabbits from both sexes and fasted prior to collection. The blood is mixed with 2.5% Na-EDTA (25ml Na-EDTA. per 250ml of the total volume). The plasma is separated from the blood cellular components by centrifugation. The plasma is pooled, bottled and stored at or below -10 °C.

Bovine Plasma Non-Sterile EDTA
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Description: Whole blood from healthy bovines that are 1 year or older is collected using 1 part 2.5% di-sodium EDTA to 9 parts blood.. The plasma is separated from the blood's cellular components by centrifugation and then stored at or below -10°C . The product was obtained from USDA inspected facilities in one of the 48 contiguous US states.

K2-EDTA Bovine Plasma filtered
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Description: K2-EDTA Bovine Plasma filtered

Trypsin-EDTA (0.25%), phenol red
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Rabbit Plasma (Sterile Sodium EDTA)
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Rabbit Plasma (Sterile Sodium EDTA)
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Sheep Plasma (Sterile Sodium EDTA)
20-abx800023
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Sheep Plasma (Sterile Sodium EDTA)
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Goat Plasma (Sterile Sodium EDTA)
20-abx800038
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Goat Plasma (Sterile Sodium EDTA)
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Horse Plasma (Sterile Sodium EDTA)
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Horse Plasma (Sterile Sodium EDTA)
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Donkey Plasma (Sterile Sodium EDTA)
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  • EUR 1957.00
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Donkey Plasma (Sterile Sodium EDTA)
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Camel Plasma (Sterile Sodium EDTA)
20-abx800083
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  • EUR 3947.00
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Camel Plasma (Sterile Sodium EDTA)
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Dog Plasma (Sterile Sodium EDTA)
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Dog Plasma (Sterile Sodium EDTA)
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Chicken Plasma (Sterile Sodium EDTA)
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Chicken Plasma (Sterile Sodium EDTA)
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EDTA disodium zinc salt hydrate
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EDTA disodium zinc salt hydrate
GX3802-250G 250 g
EUR 75

EDTA disodium zinc salt hydrate
GX3802-25G 25 g
EUR 43

EDTA disodium zinc salt hydrate
GX3802-500G 500 g
EUR 110

Constituent sulfate (SO42-) and PM2.5 from oil combustion and traffic were associated with reduced PEF. An interquartile range (IQR) increase in SO42- (3.7 μg/m3, average lags 0-1) was associated with a decrease of 0.38% (95% confidence interval = -0.75% to -0.001%). An IQR increase in oil combustion and traffic-sourced PM2.5 (2.64 μg/m3, lag 1) was associated with a decrease of 0.33% (-0.62% to -0.002%).

We found a larger PEF decrease associated with PM2.5 from dust/soil on Asian Dust days. There was no evidence linking total mass and metals to reduced pulmonary function. The ventilatory capacity of adults with severe asthma is susceptible to specific constituents/sources of PM2.5 such as sulfate and oil combustion and traffic despite active self-management of asthma and low air pollution levels in the study location.