Associations of chemical composition and sources of PM2.5 with lung function of severe asthmatic adults in a low air pollution environment of urban Nagasaki, Japan.

Previous studies have linked ambient PM2.5 to decreased pulmonary function, but the influence of specific chemical elements and emission sources on the severe asthmatic is not well understood. We examined the mass, chemical constituents, and sources of PM2.5 for short-term associations with the pulmonary function of adults with severe asthma in a low air pollution environment in urban Nagasaki, Japan. We recruited 35 asthmatic adults and obtained the daily record of morning peak expiratory flow (PEF) in spring 2014-2016. PM2.5 filters were extracted from an air quality monitoring station (178 days) and measured for 27 chemical elements. Source apportionment was performed using Positive Matrix Factorization (PMF).

We fitted generalized linear model with generalized estimating equation (GEE) method to estimate changes in PEF (from personal monthly maximum) and odds of severe respiratory deterioration (first ≥ 15% PEF reduction within a 1-week interval) associated with mass, constituents, and sources of PM2.5, with adjustment for temperature and relative humidity.

EDTA

GE4011-100G 100 g
EUR 40

EDTA

GE4011-1KG 1 kg
EUR 70

EDTA

GE4011-250G 250 g
EUR 45

EDTA

GE4011-500G 500 g
EUR 54

EDTA Solution

2103-100
EUR 137

EDTA plasma

90-1009 100 ml
EUR 2028
Description: BNP and NT-proBNP free plasma EDTA

Trypsin (-EDTA)

abx098244-100ml 100 ml
EUR 411

EDTA Na2

abx082384-500g 500 g
EUR 203

0.5M EDTA

E9000-010 100 ml
EUR 74

0.5M EDTA

E9000-050 500 ml
EUR 104

100G EDTA

NAT1090 100G
EUR 79

500G EDTA

NAT1092 500G
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TRYPSIN/EDTA 1X (0,25% TRYPSIN/2,21 MM EDTA)

25-053-CI 100 mL/pk
EUR 57
Description: Media Catalog; Cell Culture Reagents

Rabbit Plasma (EDTA)

88R-1029E 500 ml
EUR 230
Description: Sterile Rabbit Plasma with EDTA

EDTA, Free acid

EB0107 500g
EUR 66.53

100 ML TRYPSIN/EDTA 1X (0,05% TRYPSIN/0,53 MM EDTA)

25-052-CI 100 mL/pk
EUR 57
Description: Media Catalog; Cell Culture Reagents

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl

T8059-010 100ml
EUR 76

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl

T8059-050 500ml
EUR 84

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl

T8059-101 1L
EUR 126

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl

T8150-050 500 ml
EUR 84

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl

T8150-801 8L
EUR 120

Rabbit Plasma, Sterile, EDTA

31156-1 500mL
EUR 224
Description: Whole blood is collected from young (8 - 12 weeks) healthy New Zealand White or Californian rabbits from both sexes and fasted prior to collection. The blood is mixed with 2.5% Sodium EDTA (25ml Sodium EDTA per 250ml of the total volume). The plasma is separated from the blood cellular components by centrifugation. The plasma is pooled, passed through a 0.22-micron filter, bottled and stored at or below -10 °C.

DMNP-EDTA(caged calcium)

50050 5MG
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Description: Minimum order quantity: 1 unit of 5MG

Normal Feline Plasma (EDTA)

abx098254-50ml 50 ml
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Trypsin-EDTA(1X), 0.25%

CA014-010 100ml
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Trypsin-EDTA(1X), 0.25%

CA014-050 500ml
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Trypsin-EDTA(1X), 0.25%

CA014-100 10x100ml
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Trypsin-EDTA(1X), 0.25%

CA014-200 20x200ml
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Trypsin-EDTA(10X), 0.5%

CA015-010 100ml
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Trypsin-EDTA(10X), 0.5%

CA015-100 10x100ml
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Trypsin-EDTA(10X), 0.5%

CA015-200 20x100ml
EUR 482

Trypsin-EDTA(1X), 0.05%

CA020-010 100ml
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Trypsin-EDTA(1X), 0.025%

CA021-010 100ml
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Trypsin-EDTA(1X), 0.025%

CA021-050 500ml
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Trypsin-EDTA(1X), 0.025%

CA021-100 10x100ml
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Trypsin-EDTA(1X), 0.025%

CA021-200 20x100ml
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Trypsin-EDTA(10X), 0.05%

CA022-010 100ml
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EDTA, Disodium Salt, Dihydrate

CH032 500 g
EUR 163

EDTA, Disodium Salt, Dihydrate

CH033 1.0 kg
EUR 195

EDTA tripotassium salt dihydrate

GL1464-100G 100 g
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EDTA tripotassium salt dihydrate

GL1464-250G 250 g
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EDTA tripotassium salt dihydrate

GL1464-25G 25 g
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EDTA tripotassium salt dihydrate

GL1464-500G 500 g
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EDTA disodium salt dihydrate

GE3023-100G 100 g
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EDTA disodium salt dihydrate

GE3023-1KG 1 kg
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EDTA disodium salt dihydrate

GE3023-250G 250 g
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EDTA disodium salt dihydrate

GE3023-500G 500 g
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EDTA disodium salt dihydrate

GE3023-5KG 5 kg
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EDTA tetrasodium salt hydrate

GE3450-100G 100 g
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EDTA tetrasodium salt hydrate

GE3450-1KG 1 kg
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EDTA tetrasodium salt hydrate

GE3450-250G 250 g
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EDTA tetrasodium salt hydrate

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EDTA tetrasodium salt hydrate

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EDTA dipotassium salt dihydrate

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EDTA dipotassium salt dihydrate

GE5635-250G 250 g
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EDTA dipotassium salt dihydrate

GE5635-500G 500 g
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EDTA ferric sodium salt

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EDTA ferric sodium salt

GE7700-1KG 1 kg
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EDTA ferric sodium salt

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EDTA ferric sodium salt

GE7700-500G 500 g
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EDTA trisodium salt dihydrate

GE9701-100G 100 g
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EDTA trisodium salt dihydrate

GE9701-250G 250 g
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EDTA trisodium salt dihydrate

GE9701-500G 500 g
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EDTA tetrasodium salt dihydrate

GK0795-100G 100 g
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EDTA tetrasodium salt dihydrate

GK0795-1KG 1 kg
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EDTA tetrasodium salt dihydrate

GK0795-2500G 2500 g
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EDTA tetrasodium salt dihydrate

GK0795-250G 250 g
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EDTA tetrasodium salt dihydrate

GK0795-500G 500 g
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1L EDTA 0.5M Solution

NAT1282 1L
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EDTA, disodium salt, dihydrate

EB0185 500g
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EDTA, tetrasodium salt dihydrate

EB0436 500g
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EDTA, dipotassium salt, dihydrate

ED0075 250g
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EDTA 0.5M Sterile Solution

SD8135 450ml
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EDTA, Ultra Pure, 1kg

R9410-1kg
EUR 190

Rabbit Plasma Non-Sterile, EDTA

31141-1 500mL
EUR 207
Description: Whole blood is collected from young (8 - 12 weeks) healthy New Zealand White or Californian rabbits from both sexes and fasted prior to collection. The blood is mixed with 2.5% Na-EDTA (25ml Na-EDTA. per 250ml of the total volume). The plasma is separated from the blood cellular components by centrifugation. The plasma is pooled, bottled and stored at or below -10 °C.

Bovine Plasma Non-Sterile EDTA

37141-1 500mL
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Description: Whole blood from healthy bovines that are 1 year or older is collected using 1 part 2.5% di-sodium EDTA to 9 parts blood.. The plasma is separated from the blood's cellular components by centrifugation and then stored at or below -10°C . The product was obtained from USDA inspected facilities in one of the 48 contiguous US states.

K2-EDTA Bovine Plasma filtered

88R-1051 5 X 1 L
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Description: K2-EDTA Bovine Plasma filtered

Trypsin-EDTA (0.25%), phenol red

abx082465-100ml 100 ml
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Rabbit Plasma (Sterile Sodium EDTA)

20-abx800006
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  • 100 ml
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  • 50 ml

Rabbit Plasma (Sterile Sodium EDTA)

abx800006-1L 1 L
EUR 6258

Sheep Plasma (Sterile Sodium EDTA)

20-abx800023
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  • 100 ml
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Sheep Plasma (Sterile Sodium EDTA)

abx800023-1L 1 L
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Goat Plasma (Sterile Sodium EDTA)

20-abx800038
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  • EUR 829.00
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  • 100 ml
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  • 50 ml

Goat Plasma (Sterile Sodium EDTA)

abx800038-1L 1 L
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Horse Plasma (Sterile Sodium EDTA)

20-abx800053
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  • EUR 1149.00
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  • 100 ml
  • 500 ml
  • 50 ml

Horse Plasma (Sterile Sodium EDTA)

abx800053-1L 1 L
EUR 1483

Donkey Plasma (Sterile Sodium EDTA)

20-abx800068
  • EUR 1511.00
  • EUR 1957.00
  • EUR 1052.00
  • 100 ml
  • 500 ml
  • 50 ml

Donkey Plasma (Sterile Sodium EDTA)

abx800068-1L 1 L
EUR 2555

Camel Plasma (Sterile Sodium EDTA)

20-abx800083
  • EUR 1650.00
  • EUR 3947.00
  • EUR 1288.00
  • 100 ml
  • 500 ml
  • 50 ml

Camel Plasma (Sterile Sodium EDTA)

abx800083-1L 1 L
EUR 6759

Dog Plasma (Sterile Sodium EDTA)

20-abx800113
  • EUR 1400.00
  • EUR 3293.00
  • EUR 1080.00
  • 100 ml
  • 500 ml
  • 50 ml

Dog Plasma (Sterile Sodium EDTA)

abx800113-1L 1 L
EUR 5687

Chicken Plasma (Sterile Sodium EDTA)

20-abx800128
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  • EUR 1734.00
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  • 100 ml
  • 500 ml
  • 50 ml

Chicken Plasma (Sterile Sodium EDTA)

abx800128-1L 1 L
EUR 2973

EDTA disodium zinc salt hydrate

GX3802-100G 100 g
EUR 54

EDTA disodium zinc salt hydrate

GX3802-250G 250 g
EUR 75

EDTA disodium zinc salt hydrate

GX3802-25G 25 g
EUR 43

EDTA disodium zinc salt hydrate

GX3802-500G 500 g
EUR 110

Constituent sulfate (SO42-) and PM2.5 from oil combustion and traffic were associated with reduced PEF. An interquartile range (IQR) increase in SO42- (3.7 μg/m3, average lags 0-1) was associated with a decrease of 0.38% (95% confidence interval = -0.75% to -0.001%). An IQR increase in oil combustion and traffic-sourced PM2.5 (2.64 μg/m3, lag 1) was associated with a decrease of 0.33% (-0.62% to -0.002%).

We found a larger PEF decrease associated with PM2.5 from dust/soil on Asian Dust days. There was no evidence linking total mass and metals to reduced pulmonary function. The ventilatory capacity of adults with severe asthma is susceptible to specific constituents/sources of PM2.5 such as sulfate and oil combustion and traffic despite active self-management of asthma and low air pollution levels in the study location.