japan chemical inventory – Japan Chemical Week

Histopathological examination of newly-developed adhesive silicone denture relining material.

June 4, 2020May 29, 2020 by Rolf

We aimed to guage the subcutaneous tissue response to a newly developed adhesive silicone denture relining materials, SG, (Neo Dental Chemical Products Co., Ltd. Tokyo, Japan). We embedded the experimental materials SG and one other present management materials, Roeko Seal (RS), within the dorsal space of 22 male ddY mice.

One week and 12 weeks after the embedding, the tissues surrounding the embedded supplies had been eliminated and a histopathological examination was carried out. The outcomes reveal that the essential histopathological elements are the formation of granulation tissue and the change of the tissue to fibrous capsule over time.

The outcomes means that the newly-developed SG is secure as in contrast with the management RS, whose composition is comparable.

Collaborative research on fifteen compounds within the rat-liver Comet assay built-in into 2- and 4-week repeat-dose research.

A collaborative trial was carried out to guage the likelihood of integrating the rat-liver Comet assay into repeat-dose toxicity research. Fourteen laboratories from Europe, Japan and the USA examined fifteen chemical compounds.

Two chemical compounds had been beforehand proven to induce micronuclei in an acute protocol, however had been discovered unfavorable in a 4-week Micronucleus (MN) Assay (benzo[a]pyrene and 1,2-dimethylhydrazine; Hamada et al., 2001); 4 genotoxic rat-liver carcinogens that had been unfavorable within the MN assay in bone marrow or blood (2,6-dinitrotoluene, dimethylnitrosamine, 1,2-dibromomethane, and 2-amino-3-methylimidazo[4,5-f]quinoline); three compounds used within the ongoing JaCVAM (Japanese Center for the Validation of Alternative Methods) validation research of the acute liver Comet assay (2,4-diaminotoluene, 2,6-diaminotoluene and acrylamide); three pharmaceutical-like compounds (chlordiazepoxide, pyrimethamine and gemifloxacin), and three non-genotoxic rodent liver carcinogens (methapyrilene, clofibrate and phenobarbital). Male rats obtained oral administrations of the take a look at compounds, each day for 2 or 4 weeks.

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The prime dose was meant to be the very best dose producing scientific indicators or histopathological results with out inflicting mortality, i.e. the 28-day most tolerated dose. The liver Comet assay was carried out in accordance with revealed suggestions and following the protocol for the continuing JaCVAM validation trial. Laboratories supplied liver Comet assay knowledge obtained on the finish of the long-term (2- or 4-week) research along with an analysis of liver histology.

Most of the take a look at compounds had been additionally investigated within the liver Comet assay after short-term (1-Three each day) administration to match the sensitivity of the 2 research designs. MN analyses had been carried out in bone marrow or peripheral blood for many of the compounds to find out whether or not the liver Comet assay might complement the MN assay for the detection of genotoxins after long-term therapy.

Most of the liver genotoxins had been optimistic and the three non-genotoxic carcinogens gave unfavorable end result within the liver Comet assay after long-term administration. There was a excessive concordance between short- and long-term Comet assay outcomes. Most compounds when examined as much as the utmost tolerated dose had been accurately detected in each short- and long-term research.

Discrepant outcomes had been obtained with 2,6 diaminotoluene (unfavorable within the short-term, however optimistic within the long-term research), phenobarbital (optimistic within the short-term, however unfavorable within the long-term research) and gemifloxacin (optimistic within the short-term, however unfavorable within the long-term research).

The total outcomes point out that the liver Comet assay will be built-in inside repeat-dose toxicity research and effectively enhances the MN assay in detecting genotoxins. Practical elements of integrating genotoxicity endpoints into repeat-dose research had been evaluated, e.g. by investigating the impact of blood sampling, as usually carried out throughout toxicity research, on the Comet and MN assays.

The bleeding protocols used right here didn’t have an effect on the conclusions of the Comet assay or of the MN assays in blood and bone marrow. Although bleeding typically elevated reticulocyte frequencies, the sensitivity of the response within the MN assay was not altered.

FLX pyrosequencing analysis of the effects of the brown-algal fermentable polysaccharides alginate and laminaran on rat cecal microbiotas.

June 4, 2020May 29, 2020 by Rolf

Edible brown algae are used as main meals materials in Far East Asian international locations, significantly in South Korea and Japan. They comprise fermentable dietary fibers, alginic acid (uronic acid polymer) and laminaran (β-1,3-glucan), which can be fermented into natural acids by intestinal micro organism.

To make clear the impact of edible algae on the intestinal setting, the cecal microbiotas of rats fed diets containing no dietary fiber (management) or 2% (wt/wt) sodium alginate or laminaran for two weeks have been analyzed utilizing FLX amplicon pyrosequencing with bar-coded primers focusing on the bacterial 16S rRNA gene. The most considerable phylum in all teams was Firmicutes.

Specifically, Allobaculum was dominant in all food regimen teams. In addition, Bacteroides capillosus (37.1%) was considerable in the alginate group, whereas Clostridium ramosum (3.14%) and Parabacteroides distasonis (1.36%) have been solely detected in the laminaran group. Furthermore, rats fed alginate confirmed simplified microbiota phylotypes in contrast with others. With respect to cecal chemical compounds, laminaran increased cecal natural acid ranges, significantly propionic acid. Alginate elevated complete cecal natural acids.

Cecal putrefactive compounds, comparable to indole, H(2)S, and phenol, have been decreased by each alginate and laminaran. These outcomes point out that edible brown algae can alter the intestinal setting, with fermentation by intestinal microbiota.

Tsunami lung.

We encountered three instances of lung issues attributable to drowning in the current massive tsunami that struck following the Great East Japan Earthquake. All three have been females, and two of them have been outdated aged. All segments of each lungs have been concerned in all the three sufferers, necessitating ICU admission and endotracheal intubation and mechanical air flow. All three died inside 3 weeks.

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In no less than two instances, misswallowing of oil was suspected from the options famous at the time of the detection. Sputum tradition for micro organism yielded isolation of Stenotrophomonas maltophilia, Legionella pneumophila, Burkholderia cepacia, and Pseudomonas aeruginosa. The trigger of tsunami lung could also be a mix of chemical induced pneumonia and bacterial pneumonia.

Cultivated oral mucosal epithelial transplantation for persistent epithelial defect in severe ocular surface diseases with acute inflammatory activity.

June 4, 2020May 29, 2020 by Rolf

OBJECTIVE

To assess the medical efficacy of cultivated oral mucosal epithelial transplantation (COMET) for the remedy of persistent epithelial defect (PED).

METHODS

We handled 10 eyes of 9 sufferers with PED (Stevens-Johnson syndrome: three eyes; thermal/chemical harm: 5 eyes; ocular cicatricial pemphigoid: two eyes) with COMET at Kyoto Prefectural University of Medicine, Kyoto, Japan from 2002 to 2008.

RESULTS

Preoperatively, PED existed on over greater than 50% of the corneal surface in seven eyes. Severe ocular surface irritation with fibrovascular tissue surrounded the PED in all 10 eyes. At 24-weeks postoperative, PED had improved in all instances besides 1 in which the affected person was unable to return to the hospital (95% CI, 55.5-99.7; Wilcoxon signed-rank check, p = 0.0078).

The preoperative median of logarithmic minimal angle of decision was 1.85 (vary 0.15-2.70), and 1.85, 1.85, and 1.52 on the 4th, 12th, and 24th postoperative week, respectively.

The imply whole preoperative ocular surface grading rating was 7.0 (vary 4-17). At Four and 12 weeks postoperative, the whole ocular surface grading rating had improved considerably (p = 0.0020, p = 0.0078), and at 24 weeks postoperative, it was 3.0 (vary 2-12, p = 0.0234). During the follow-up interval (median 23.Three months, vary 5.6-39.7 months), no recurrence of PED was noticed in any eye, and long-term ocular surface stability was obtained.

CONCLUSIONS

COMET enabled full epithelialization of PED and stabilization of the ocular surface in sufferers with severe ocular surface illness, thus stopping end-stage cicatrization and imaginative and prescient loss at a later stage.

Antianxiety-like results of Chimpi (dried citrus peels) in the elevated open-platform check.

Dried citrus peels (Chimpi) is likely one of the most typical pure medicines with qi (power circulate) rectifying and shi (dampness) drying actions, which originates from Citrus unshiu, and/or C. reticulata in response to the definition of the pharmacopoeiae of Japan and China. In this examine, the pharmacological results of their extracts and main chemical constituents hesperidin and its aglycone hesperetin on nervousness had been examined with an nervousness mannequin of elevated open-platform check utilizing ICR male mice (6-week-old) and whole period of freezing was decreased in fluoxetine-treated mice, which is a straightforward and extremely delicate to the consequences of serotonergic anxiolytics.

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Moreover, yokukansankachimpihange (YKH), a mix of yokukansan with Chimpi and Hange (Pinellia) was additionally examined as a result of Chimpi is taken into account to play an important half in this formulation in opposition to anxious signs in dementia sufferers.

The outcomes confirmed that Chimpi and YKH possess a major anxiolytic-like impact much like that of fluoxetine, suggesting that they could be much like fluoxetine in their pharmacological actions by way of the serotonergic neurotransmission pathway.

Moreover, it additionally advised that the main chemical constituent, hesperidin may very well be an lively precept attributed to the antianxiety-like results with a direct and oblique position through its aglycone hesperetin.