Anthracnose caused by Glomerella cingulata is one of the most devastating diseases of strawberry in Japan, particularly during its nursery period in the summer. In this study, we aimed to isolate and screen endophytic actinobacteria, to identify potential biocontrol agents capable of suppressing strawberry anthracnose.
A total of 226 actinobacteria were successfully isolated from surface-sterilized strawberry tissues. In the first screening, 217 out of 226 actinobacteria isolates were studied for their suppression effect on strawberry anthracnose using a detached leaflet assay. It was discovered that isolates MBFA-172 and MBFA-227 markedly suppressed the development of anthracnose lesions.
The efficacy of both isolates was then tested on two-month-old strawberry plug seedlings in a controlled environmental chamber. It was found that isolate MBFA-172 provided consistent disease suppression and was thus selected as a final candidate for further evaluation in a glasshouse experiment. Results showed that the severity as well as incidence rate of strawberry anthracnose was significantly reduced by treatment with isolate MBFA-172 compared with that of untreated control.
Accordingly, the disease control efficacy provided by MBFA-172 was statistically comparable to the chemical fungicide propineb. A re-isolation experiment using a spontaneous thiostrepton-resistant mutated strain of isolate MBFA-172 revealed that it efficiently colonized the above-ground tissues of strawberry plants for at least three weeks after spray treatment.
AI9200 Melting Point Meter |
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MEL1010 | Scientific Laboratory Supplies | EACH | EUR 1634.76 |
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EDTA |
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B7197-1000 | ApexBio | 1 g | EUR 154.8 |
EDTA |
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GE4011-100G | Glentham Life Sciences | 100 g | EUR 48 |
EDTA |
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GE4011-1KG | Glentham Life Sciences | 1 kg | EUR 84 |
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19010 | AAT Bioquest | 10x50 ug | EUR 109 |
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19011 | AAT Bioquest | 10 mg | EUR 166 |
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Trypsin-EDTA(1X), 0.05% |
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Trypsin-EDTA(1X), 0.025% |
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EDTA disodium salt dihydrate |
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EDTA dipotassium salt dihydrate |
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EDTA trisodium salt dihydrate |
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240827 | Scientific Laboratory Supplies | PK10 | EUR 76.38 |
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37141-1 | Pel-Freez | 500mL | EUR 221.56 |
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39541-1 | Pel-Freez | 500mL | EUR 221.56 |
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31141-1 | Pel-Freez | 500mL | EUR 314.21 |
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31156-1 | Pel-Freez | 500mL | EUR 342.91 |
CK PLASMA NS EDTA 500mL |
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33141-1 | Pel-Freez | 500mL | EUR 221.56 |
Trypsin-EDTA (0.25%), phenol red |
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abx082465-100ml | Abbexa | 100 ml | EUR 243.6 |
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Rabbit Plasma (Sterile Sodium EDTA) |
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abx800006-1L | Abbexa | 1 L | EUR 7509.6 |
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20-abx800023 | Abbexa |
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Sheep Plasma (Sterile Sodium EDTA) |
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abx800023-1L | Abbexa | 1 L | EUR 1195.2 |
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20-abx800038 | Abbexa |
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abx800038-1L | Abbexa | 1 L | EUR 1195.2 |
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20-abx800053 | Abbexa |
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Horse Plasma (Sterile Sodium EDTA) |
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abx800053-1L | Abbexa | 1 L | EUR 1779.6 |
Donkey Plasma (Sterile Sodium EDTA) |
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20-abx800068 | Abbexa |
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Donkey Plasma (Sterile Sodium EDTA) |
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abx800068-1L | Abbexa | 1 L | EUR 3066 |
Camel Plasma (Sterile Sodium EDTA) |
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20-abx800083 | Abbexa |
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Camel Plasma (Sterile Sodium EDTA) |
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abx800083-1L | Abbexa | 1 L | EUR 8110.8 |
Dog Plasma (Sterile Sodium EDTA) |
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20-abx800113 | Abbexa |
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Dog Plasma (Sterile Sodium EDTA) |
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abx800113-1L | Abbexa | 1 L | EUR 6824.4 |
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20-abx800128 | Abbexa |
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Chicken Plasma (Sterile Sodium EDTA) |
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abx800128-1L | Abbexa | 1 L | EUR 3567.6 |
K2-EDTA Bovine Plasma filtered |
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88R-1051 | Fitzgerald | 5 X 1 L | EUR 1980 |
Description: K2-EDTA Bovine Plasma filtered |
EDTA copper(II) disodium salt |
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EB0434 | Bio Basic | 250g | EUR 100.72 |
EDTA disodium and monomagnesium salt |
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EB0435 | Bio Basic | 500g | EUR 133.08 |
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EB0437 | Bio Basic | 100g | EUR 78.79 |
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EB6666 | Bio Basic | 100g | EUR 77.75 |
Protease Inhibition Cocktail with EDTA |
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G135E | ABM | 1.0 ml | EUR 200.4 |
0.5 M EDTA, pH 8.0 |
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GR103066 | Genorise Scientific | 100 mL | EUR 82.8 |
EDTA disodium zinc salt hydrate |
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GX3802-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
EDTA disodium zinc salt hydrate |
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GX3802-250G | Glentham Life Sciences | 250 g | EUR 90 |
EDTA disodium zinc salt hydrate |
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GX3802-25G | Glentham Life Sciences | 25 g | EUR 51.6 |
EDTA disodium zinc salt hydrate |
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GX3802-500G | Glentham Life Sciences | 500 g | EUR 132 |
EDTA manganese disodium salt hydrate |
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GX6009-100G | Glentham Life Sciences | 100 g | EUR 122.4 |
EDTA manganese disodium salt hydrate |
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GX6009-250G | Glentham Life Sciences | 250 g | EUR 208.8 |
EDTA manganese disodium salt hydrate |
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GX6009-25G | Glentham Life Sciences | 25 g | EUR 69.6 |
EDTA manganese disodium salt hydrate |
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GX6009-500G | Glentham Life Sciences | 500 g | EUR 322.8 |
Using cultural, morphological, and physiological tests combined with 16S rRNA-based molecular analysis, MBFA-172 was identified as a moderately thermophilic Streptomyces thermocarboxydus-related species. Upon review, our results strongly indicated that MBFA-172 is a promising biocontrol agent for strawberry anthracnose.