A novel strain of endophytic Streptomyces for the biocontrol of strawberry anthracnose caused by Glomerella cingulata.

Anthracnose caused by Glomerella cingulata is one of the most devastating diseases of strawberry in Japan, particularly during its nursery period in the summer. In this study, we aimed to isolate and screen endophytic actinobacteria, to identify potential biocontrol agents capable of suppressing strawberry anthracnose.

A total of 226 actinobacteria were successfully isolated from surface-sterilized strawberry tissues. In the first screening, 217 out of 226 actinobacteria isolates were studied for their suppression effect on strawberry anthracnose using a detached leaflet assay. It was discovered that isolates MBFA-172 and MBFA-227 markedly suppressed the development of anthracnose lesions.

The efficacy of both isolates was then tested on two-month-old strawberry plug seedlings in a controlled environmental chamber. It was found that isolate MBFA-172 provided consistent disease suppression and was thus selected as a final candidate for further evaluation in a glasshouse experiment. Results showed that the severity as well as incidence rate of strawberry anthracnose was significantly reduced by treatment with isolate MBFA-172 compared with that of untreated control.

Accordingly, the disease control efficacy provided by MBFA-172 was statistically comparable to the chemical fungicide propineb. A re-isolation experiment using a spontaneous thiostrepton-resistant mutated strain of isolate MBFA-172 revealed that it efficiently colonized the above-ground tissues of strawberry plants for at least three weeks after spray treatment.

HiQTM Low Melting Agarose
A0223-010 100g
EUR 583

Agarose, Low melting gel
AB0015 10g
EUR 80.45
  • Product category: Electrophoresis Related/Agarose

EDTA
B7197-1000 1 g
EUR 129

EDTA
GE4011-100G 100 g
EUR 40

EDTA
GE4011-1KG 1 kg
EUR 70

EDTA
GE4011-250G 250 g
EUR 45

EDTA
GE4011-500G 500 g
EUR 54

EDTA
20-abx082228
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HyAgarose? LM Agarose, Low Melting, 100g
R9012LM-100g
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R9012LM-10g
EUR 100

HyAgarose? LM Agarose, Low Melting, 500g
R9012LM-500g
EUR 1518

HyAgarose? LM Agarose, Low Melting, 50g
R9012LM-50g
EUR 219

0.5M EDTA
E9000-010 100 ml
EUR 74

0.5M EDTA
E9000-050 500 ml
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EDTA Na2
abx082384-500g 500 g
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Trypsin (-EDTA)
abx098244-100ml 100 ml
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EDTA plasma
90-1009 100 ml
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Description: BNP and NT-proBNP free plasma EDTA

EDTA Solution
2103-100
EUR 137

100G EDTA
NAT1090 100G
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500G EDTA
NAT1092 500G
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TRYPSIN/EDTA 1X (0,25% TRYPSIN/2,21 MM EDTA)
25-053-CI 100 mL/pk
EUR 57
Description: Media Catalog; Cell Culture Reagents

Rabbit Plasma (EDTA)
88R-1029E 500 ml
EUR 230
Description: Sterile Rabbit Plasma with EDTA

EDTA, Free acid
EB0107 500g
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45UL FINE POINT TIPS FOR BECKMAN FX ROBOTICS SYSTEM,
FX-384-45FP-R 3840/pk
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30UL FINE POINT MAXYMUM RECOVERY TIP. 384 TIPS PER RACK
VT-384-31-L-R 3840/pk
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Description: Robotic Tips; Maxymum Recovery Robotic Tips - Axygen

100 ML TRYPSIN/EDTA 1X (0,05% TRYPSIN/0,53 MM EDTA)
25-052-CI 100 mL/pk
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Description: Media Catalog; Cell Culture Reagents

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-010 100ml
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Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-050 500ml
EUR 84

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-101 1L
EUR 126

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8150-050 500 ml
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Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8150-801 8L
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Trypsin-EDTA(1X), 0.25%
CA014-010 100ml
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Trypsin-EDTA(1X), 0.25%
CA014-050 500ml
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Trypsin-EDTA(1X), 0.25%
CA014-100 10x100ml
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Trypsin-EDTA(1X), 0.25%
CA014-200 20x200ml
EUR 354

Trypsin-EDTA(10X), 0.5%
CA015-010 100ml
EUR 88

Trypsin-EDTA(10X), 0.5%
CA015-100 10x100ml
EUR 286

Trypsin-EDTA(10X), 0.5%
CA015-200 20x100ml
EUR 482

Trypsin-EDTA(1X), 0.05%
CA020-010 100ml
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Trypsin-EDTA(1X), 0.025%
CA021-010 100ml
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Trypsin-EDTA(1X), 0.025%
CA021-050 500ml
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Trypsin-EDTA(1X), 0.025%
CA021-100 10x100ml
EUR 223

Trypsin-EDTA(1X), 0.025%
CA021-200 20x100ml
EUR 334

Trypsin-EDTA(10X), 0.05%
CA022-010 100ml
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EDTA tripotassium salt dihydrate
GL1464-100G 100 g
EUR 61

EDTA tripotassium salt dihydrate
GL1464-250G 250 g
EUR 86

EDTA tripotassium salt dihydrate
GL1464-25G 25 g
EUR 44

EDTA tripotassium salt dihydrate
GL1464-500G 500 g
EUR 128

EDTA disodium salt dihydrate
GE3023-100G 100 g
EUR 40

EDTA disodium salt dihydrate
GE3023-1KG 1 kg
EUR 70

EDTA disodium salt dihydrate
GE3023-250G 250 g
EUR 46

EDTA disodium salt dihydrate
GE3023-500G 500 g
EUR 54

EDTA disodium salt dihydrate
GE3023-5KG 5 kg
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EDTA tetrasodium salt hydrate
GE3450-100G 100 g
EUR 41

EDTA tetrasodium salt hydrate
GE3450-1KG 1 kg
EUR 68

EDTA tetrasodium salt hydrate
GE3450-250G 250 g
EUR 45

EDTA tetrasodium salt hydrate
GE3450-500G 500 g
EUR 53

EDTA tetrasodium salt hydrate
GE3450-5KG 5 kg
EUR 166

EDTA dipotassium salt dihydrate
GE5635-100G 100 g
EUR 54

EDTA dipotassium salt dihydrate
GE5635-250G 250 g
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EDTA dipotassium salt dihydrate
GE5635-500G 500 g
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GE7700-100G 100 g
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EDTA ferric sodium salt
GE7700-1KG 1 kg
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EDTA ferric sodium salt
GE7700-250G 250 g
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EDTA ferric sodium salt
GE7700-500G 500 g
EUR 70

EDTA trisodium salt dihydrate
GE9701-100G 100 g
EUR 54

EDTA trisodium salt dihydrate
GE9701-250G 250 g
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EDTA trisodium salt dihydrate
GE9701-500G 500 g
EUR 118

EDTA tetrasodium salt dihydrate
GK0795-100G 100 g
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EDTA tetrasodium salt dihydrate
GK0795-1KG 1 kg
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EDTA tetrasodium salt dihydrate
GK0795-2500G 2500 g
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EDTA tetrasodium salt dihydrate
GK0795-250G 250 g
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GK0795-500G 500 g
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Description: Whole blood is collected from young (8 - 12 weeks) healthy New Zealand White or Californian rabbits from both sexes and fasted prior to collection. The blood is mixed with 2.5% Sodium EDTA (25ml Sodium EDTA per 250ml of the total volume). The plasma is separated from the blood cellular components by centrifugation. The plasma is pooled, passed through a 0.22-micron filter, bottled and stored at or below -10 °C.

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CH032 500 g
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EDTA, Disodium Salt, Dihydrate
CH033 1.0 kg
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1L EDTA 0.5M Solution
NAT1282 1L
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R9410-1kg
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SD8135 450ml
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EDTA, disodium salt, dihydrate
EB0185 500g
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EB0436 500g
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EDTA, dipotassium salt, dihydrate
ED0075 250g
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  • Product category: Biochemicals/Misc. Biochemicals

30UL FINE POINT MAXYMUM RECOVERY TIP, STERILE. 384 TIPS PER RACK
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Description: Robotic Tips; Filter Tips Robotic Tips - Axygen

Rabbit Plasma (Sterile Sodium EDTA)
20-abx800006
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Rabbit Plasma (Sterile Sodium EDTA)
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Sheep Plasma (Sterile Sodium EDTA)
20-abx800023
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Sheep Plasma (Sterile Sodium EDTA)
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Goat Plasma (Sterile Sodium EDTA)
20-abx800038
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Goat Plasma (Sterile Sodium EDTA)
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Horse Plasma (Sterile Sodium EDTA)
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Horse Plasma (Sterile Sodium EDTA)
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Donkey Plasma (Sterile Sodium EDTA)
20-abx800068
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Donkey Plasma (Sterile Sodium EDTA)
abx800068-1L 1 L
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Camel Plasma (Sterile Sodium EDTA)
20-abx800083
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Camel Plasma (Sterile Sodium EDTA)
abx800083-1L 1 L
EUR 6759
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Dog Plasma (Sterile Sodium EDTA)
20-abx800113
  • EUR 1400.00
  • EUR 3293.00
  • EUR 1080.00
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Dog Plasma (Sterile Sodium EDTA)
abx800113-1L 1 L
EUR 5687
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Chicken Plasma (Sterile Sodium EDTA)
20-abx800128
  • EUR 815.00
  • EUR 1734.00
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Chicken Plasma (Sterile Sodium EDTA)
abx800128-1L 1 L
EUR 2973
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EDTA disodium zinc salt hydrate
GX3802-100G 100 g
EUR 54

EDTA disodium zinc salt hydrate
GX3802-250G 250 g
EUR 75

EDTA disodium zinc salt hydrate
GX3802-25G 25 g
EUR 43

EDTA disodium zinc salt hydrate
GX3802-500G 500 g
EUR 110

EDTA manganese disodium salt hydrate
GX6009-100G 100 g
EUR 102

EDTA manganese disodium salt hydrate
GX6009-250G 250 g
EUR 174

EDTA manganese disodium salt hydrate
GX6009-25G 25 g
EUR 58

EDTA manganese disodium salt hydrate
GX6009-500G 500 g
EUR 269

EDTA magnesium disodium salt hydrate
GE3956-100G 100 g
EUR 66

EDTA magnesium disodium salt hydrate
GE3956-1KG 1 kg
EUR 245

EDTA magnesium disodium salt hydrate
GE3956-250G 250 g
EUR 102

EDTA magnesium disodium salt hydrate
GE3956-25G 25 g
EUR 45

EDTA magnesium disodium salt hydrate
GE3956-500G 500 g
EUR 154

Protease Inhibition Cocktail with EDTA
G135E 1.0 ml
EUR 167

Trypsin-EDTA (0.25%), phenol red
abx082465-100ml 100 ml
EUR 203
  • Shipped within 5-10 working days.

K2-EDTA Bovine Plasma filtered
88R-1051 5 X 1 L
EUR 1650
Description: K2-EDTA Bovine Plasma filtered

Bovine Plasma Non-Sterile EDTA
37141-1 500mL
EUR 178
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Description: Whole blood from healthy bovines that are 1 year or older is collected using 1 part 2.5% di-sodium EDTA to 9 parts blood.. The plasma is separated from the blood's cellular components by centrifugation and then stored at or below -10°C . The product was obtained from USDA inspected facilities in one of the 48 contiguous US states.

Rabbit Plasma Non-Sterile, EDTA
31141-1 500mL
EUR 207
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Description: Whole blood is collected from young (8 - 12 weeks) healthy New Zealand White or Californian rabbits from both sexes and fasted prior to collection. The blood is mixed with 2.5% Na-EDTA (25ml Na-EDTA. per 250ml of the total volume). The plasma is separated from the blood cellular components by centrifugation. The plasma is pooled, bottled and stored at or below -10 °C.

EDTA copper(II) disodium salt
EB0434 250g
EUR 83.93
  • Product category: Biochemicals/Misc. Biochemicals

EDTA disodium and monomagnesium salt
EB0435 500g
EUR 110.9
  • Product category: Biochemicals/Misc. Biochemicals

EDTA, Iron (III), sodium salt
EB0437 100g
EUR 65.66
  • Product category: Biochemicals/Misc. Biochemicals

EDTA zinc (II), disodium salt
EB6666 100g
EUR 64.79
  • Product category: Biochemicals/Misc. Biochemicals

EDTA disodium and monocalcium salt
EK014P2 500g
EUR 96.11
  • Product category: Biochemicals/Misc. Biochemicals

30UL PRE-STERILIZED FILTERED FINE POINT TIPS FOR BECKMAN FX ROBOTICS SYSTEM
FXF-384-30FP-R-S 3840/pk
EUR 4777
Description: Robotic Tips; Filter Tips Robotic Tips - Axygen

Non-sterile canine plasma with EDTA
CAPE07-0100 100 ml
EUR 300.3
  • Non-sterile canine plasma with EDTA is indicated as RUO. Do not use on humans.

Non-sterile canine plasma with EDTA
CAPE07-0500 500 ml
EUR 556.4
  • Non-sterile canine plasma with EDTA is indicated as RUO. Do not use on humans.

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)
CE135 250 g
EUR 60

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)
CE136 500 g
EUR 72

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)
CE137 1 kg
EUR 104

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)
CE138 5 kg
EUR 349

Rabbit Plasma (Non-Sterile Sodium EDTA)
20-abx800005
  • EUR 1191.00
  • EUR 2806.00
  • EUR 746.00
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Rabbit Plasma (Non-Sterile Sodium EDTA)
abx800005-1L 1 L
EUR 4782
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Rabbit Whole Blood (Sterile Sodium EDTA)
20-abx800012
  • EUR 578.00
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Sheep Plasma (Non-Sterile Sodium EDTA)
20-abx800022
  • EUR 537.00
  • EUR 662.00
  • EUR 467.00
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Sheep Plasma (Non-Sterile Sodium EDTA)
abx800022-10L 10 L
EUR 4782
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Sheep Whole Blood (Sterile Sodium EDTA)
20-abx800029
  • EUR 314.00
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Goat Plasma (Non-Sterile Sodium EDTA)
20-abx800037
  • EUR 537.00
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Goat Plasma (Non-Sterile Sodium EDTA)
abx800037-1L 1 L
EUR 829
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Goat Whole Blood (Sterile Sodium EDTA)
20-abx800044
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Horse Plasma (Non-Sterile Sodium EDTA)
20-abx800052
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Horse Plasma (Non-Sterile Sodium EDTA)
abx800052-1L 1 L
EUR 1149
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Horse Whole Blood (Sterile Sodium EDTA)
20-abx800059
  • EUR 425.00
  • EUR 578.00
  • EUR 356.00
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Horse Whole Blood (Sterile Sodium EDTA)
abx800059-1L 1 L
EUR 690
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Donkey Plasma (Non-Sterile Sodium EDTA)
20-abx800067
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Donkey Plasma (Non-Sterile Sodium EDTA)
abx800067-1L 1 L
EUR 1957
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Donkey Whole Blood (Sterile Sodium EDTA)
20-abx800074
  • EUR 648.00
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  • EUR 467.00
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Donkey Whole Blood (Sterile Sodium EDTA)
abx800074-1L 1 L
EUR 1094
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Camel Plasma (Non-Sterile Sodium EDTA)
20-abx800082
  • EUR 1288.00
  • EUR 3042.00
  • EUR 1024.00
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Camel Plasma (Non-Sterile Sodium EDTA)
abx800082-1L 1 L
EUR 5102
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Camel Whole Blood (Sterile Sodium EDTA)
20-abx800089
  • EUR 718.00
  • EUR 1748.00
  • EUR 537.00
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Camel Whole Blood (Sterile Sodium EDTA)
abx800089-1L 1 L
EUR 2736
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Beagle dog Plasma (Sterile Sodium EDTA)
20-abx800098
  • EUR 2124.00
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  • EUR 1567.00
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Beagle dog Plasma (Sterile Sodium EDTA)
abx800098-1L 1 L
EUR 8722
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Dog Plasma (Non-Sterile Sodium EDTA)
20-abx800112
  • EUR 1094.00
  • EUR 2555.00
  • EUR 829.00
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Dog Plasma (Non-Sterile Sodium EDTA)
abx800112-1L 1 L
EUR 4281
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Dog Whole Blood (Sterile Sodium EDTA)
20-abx800119
  • EUR 620.00
  • EUR 439.00
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Chicken Plasma (Non-Sterile Sodium EDTA)
20-abx800127
  • EUR 634.00
  • EUR 1400.00
  • EUR 495.00
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Chicken Plasma (Non-Sterile Sodium EDTA)
abx800127-1L 1 L
EUR 2291
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Using cultural, morphological, and physiological tests combined with 16S rRNA-based molecular analysis, MBFA-172 was identified as a moderately thermophilic Streptomyces thermocarboxydus-related species. Upon review, our results strongly indicated that MBFA-172 is a promising biocontrol agent for strawberry anthracnose.