Anthracnose caused by Glomerella cingulata is one of the most devastating diseases of strawberry in Japan, particularly during its nursery period in the summer. In this study, we aimed to isolate and screen endophytic actinobacteria, to identify potential biocontrol agents capable of suppressing strawberry anthracnose.
A total of 226 actinobacteria were successfully isolated from surface-sterilized strawberry tissues. In the first screening, 217 out of 226 actinobacteria isolates were studied for their suppression effect on strawberry anthracnose using a detached leaflet assay. It was discovered that isolates MBFA-172 and MBFA-227 markedly suppressed the development of anthracnose lesions.
The efficacy of both isolates was then tested on two-month-old strawberry plug seedlings in a controlled environmental chamber. It was found that isolate MBFA-172 provided consistent disease suppression and was thus selected as a final candidate for further evaluation in a glasshouse experiment. Results showed that the severity as well as incidence rate of strawberry anthracnose was significantly reduced by treatment with isolate MBFA-172 compared with that of untreated control.
Accordingly, the disease control efficacy provided by MBFA-172 was statistically comparable to the chemical fungicide propineb. A re-isolation experiment using a spontaneous thiostrepton-resistant mutated strain of isolate MBFA-172 revealed that it efficiently colonized the above-ground tissues of strawberry plants for at least three weeks after spray treatment.
AI9100 Melting Point Meter |
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AB0015 | Bio Basic | 10g | EUR 96.54 |
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A0223-002 | GenDepot | 25g | EUR 343.2 |
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HyAgarose? LM Agarose, Low Melting, 100g |
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R9012LM-100g | ACTGene | each | EUR 452.4 |
HyAgarose? LM Agarose, Low Melting, 500g |
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HyAgarose? LM Agarose, Low Melting, 10g |
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R9012LM-50g | ACTGene | each | EUR 262.8 |
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AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL) |
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Point Sampler - 1000mm - 316ss |
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SAM1057 | Scientific Laboratory Supplies | EACH | EUR 1306.8 |
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Needle Rn Gauge 29 Type Point and Length Specify |
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DD75625 | Scientific Laboratory Supplies | PK6 | EUR 547.2 |
45UL FINE POINT TIPS FOR BECKMAN FX ROBOTICS SYSTEM, |
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FX-384-45FP-R | CORNING | 3840/pk | EUR 3241.2 |
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30UL FINE POINT MAXYMUM RECOVERY TIP. 384 TIPS PER RACK |
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VT-384-31-L-R | CORNING | 3840/pk | EUR 3218.4 |
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30UL FINE POINT MAXYMUM RECOVERY TIP, STERILE. 384 TIPS PER RACK |
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EDTA |
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B7197-1000 | ApexBio | 1 g | EUR 154.8 |
EDTA |
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20-abx082228 | Abbexa |
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EDTA |
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GE4011-100G | Glentham Life Sciences | 100 g | EUR 48 |
EDTA |
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GE4011-1KG | Glentham Life Sciences | 1 kg | EUR 84 |
EDTA |
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GE4011-250G | Glentham Life Sciences | 250 g | EUR 54 |
EDTA |
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EDTA |
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GE4011-1 | Glentham Life Sciences | 1 | EUR 39.4 |
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GE4011-100 | Glentham Life Sciences | 100 | EUR 11.3 |
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30UL PRE-STERILIZED FILTERED FINE POINT TIPS FOR BECKMAN FX ROBOTICS SYSTEM |
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0.4mL clear conical glass insert; with pulled point ; Level 3 High Performance Applications |
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0.4mL clear conical glass insert; with pulled point ; Level 3 High Performance Applications |
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0.4mL clear conical glass insert; with pulled point ; Level 3 High Performance Applications |
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0.5M EDTA |
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0.5M EDTA |
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E9000-010 | GenDepot | 100 ml | EUR 88.8 |
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EDTA Solution |
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2103-100 | Biovision | each | EUR 164.4 |
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NAT1282 | Scientific Laboratory Supplies | 1L | EUR 183.6 |
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0.5 M EDTA, pH 8.0 |
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EDTA, Disodium Salt, Dihydrate |
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EDTA, Disodium Salt, Dihydrate |
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EDTA disodium salt dihydrate |
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EDTA disodium salt dihydrate |
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EDTA disodium salt dihydrate |
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EDTA disodium salt dihydrate |
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EDTA, disodium salt, dihydrate |
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EDTA disodium salt dihydrate |
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EDTA disodium salt dihydrate |
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EDTA disodium salt dihydrate |
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GE3023-250 | Glentham Life Sciences | 250 | EUR 15.9 |
EDTA disodium salt dihydrate |
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EDTA disodium salt dihydrate |
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EDTA tetrasodium salt hydrate |
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GE3450-100G | Glentham Life Sciences | 100 g | EUR 49.2 |
EDTA tetrasodium salt hydrate |
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GE3450-1KG | Glentham Life Sciences | 1 kg | EUR 81.6 |
EDTA tetrasodium salt hydrate |
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GE3450-250G | Glentham Life Sciences | 250 g | EUR 54 |
EDTA tetrasodium salt hydrate |
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GE3450-500G | Glentham Life Sciences | 500 g | EUR 63.6 |
EDTA tetrasodium salt hydrate |
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GE3450-5KG | Glentham Life Sciences | 5 kg | EUR 199.2 |
EDTA trisodium salt dihydrate |
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GE9701-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
EDTA trisodium salt dihydrate |
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GE9701-250G | Glentham Life Sciences | 250 g | EUR 93.6 |
EDTA trisodium salt dihydrate |
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GE9701-500G | Glentham Life Sciences | 500 g | EUR 141.6 |
EDTA tetrasodium salt hydrate |
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GE3450-1 | Glentham Life Sciences | 1 | EUR 38 |
EDTA tetrasodium salt hydrate |
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GE3450-100 | Glentham Life Sciences | 100 | EUR 11.8 |
EDTA tetrasodium salt hydrate |
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GE3450-250 | Glentham Life Sciences | 250 | EUR 16.4 |
EDTA tetrasodium salt hydrate |
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GE3450-5 | Glentham Life Sciences | 5 | EUR 134.4 |
EDTA tetrasodium salt hydrate |
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GE3450-500 | Glentham Life Sciences | 500 | EUR 22.9 |
EDTA trisodium salt dihydrate |
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GE9701-100 | Glentham Life Sciences | 100 | EUR 23.8 |
EDTA trisodium salt dihydrate |
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GE9701-250 | Glentham Life Sciences | 250 | EUR 47.4 |
EDTA trisodium salt dihydrate |
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GE9701-500 | Glentham Life Sciences | 500 | EUR 87.1 |
EDTA Solution 1.62% w/v |
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EDTA1625 | Scientific Laboratory Supplies | 5L | EUR 180 |
EDTA tetrasodium salt dihydrate |
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GK0795-100G | Glentham Life Sciences | 100 g | EUR 48 |
EDTA tetrasodium salt dihydrate |
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GK0795-1KG | Glentham Life Sciences | 1 kg | EUR 84 |
EDTA tetrasodium salt dihydrate |
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GK0795-2500G | Glentham Life Sciences | 2500 g | EUR 141.6 |
EDTA tetrasodium salt dihydrate |
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GK0795-250G | Glentham Life Sciences | 250 g | EUR 55.2 |
EDTA tetrasodium salt dihydrate |
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GK0795-500G | Glentham Life Sciences | 500 g | EUR 63.6 |
EDTA dipotassium salt dihydrate |
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GE5635-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
EDTA dipotassium salt dihydrate |
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GE5635-250G | Glentham Life Sciences | 250 g | EUR 88.8 |
EDTA dipotassium salt dihydrate |
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GE5635-500G | Glentham Life Sciences | 500 g | EUR 122.4 |
EDTA, tetrasodium salt dihydrate |
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EB0436 | Bio Basic | 500g | EUR 105.94 |
EDTA, dipotassium salt, dihydrate |
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ED0075 | Bio Basic | 250g | EUR 99.67 |
EDTA dipotassium salt dihydrate |
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GE5635-100 | Glentham Life Sciences | 100 | EUR 23.8 |
EDTA dipotassium salt dihydrate |
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GE5635-250 | Glentham Life Sciences | 250 | EUR 43.5 |
EDTA dipotassium salt dihydrate |
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GE5635-500 | Glentham Life Sciences | 500 | EUR 71.1 |
Using cultural, morphological, and physiological tests combined with 16S rRNA-based molecular analysis, MBFA-172 was identified as a moderately thermophilic Streptomyces thermocarboxydus-related species. Upon review, our results strongly indicated that MBFA-172 is a promising biocontrol agent for strawberry anthracnose.