A novel strain of endophytic Streptomyces for the biocontrol of strawberry anthracnose caused by Glomerella cingulata.

Anthracnose caused by Glomerella cingulata is one of the most devastating diseases of strawberry in Japan, particularly during its nursery period in the summer. In this study, we aimed to isolate and screen endophytic actinobacteria, to identify potential biocontrol agents capable of suppressing strawberry anthracnose.

A total of 226 actinobacteria were successfully isolated from surface-sterilized strawberry tissues. In the first screening, 217 out of 226 actinobacteria isolates were studied for their suppression effect on strawberry anthracnose using a detached leaflet assay. It was discovered that isolates MBFA-172 and MBFA-227 markedly suppressed the development of anthracnose lesions.

The efficacy of both isolates was then tested on two-month-old strawberry plug seedlings in a controlled environmental chamber. It was found that isolate MBFA-172 provided consistent disease suppression and was thus selected as a final candidate for further evaluation in a glasshouse experiment. Results showed that the severity as well as incidence rate of strawberry anthracnose was significantly reduced by treatment with isolate MBFA-172 compared with that of untreated control.

Accordingly, the disease control efficacy provided by MBFA-172 was statistically comparable to the chemical fungicide propineb. A re-isolation experiment using a spontaneous thiostrepton-resistant mutated strain of isolate MBFA-172 revealed that it efficiently colonized the above-ground tissues of strawberry plants for at least three weeks after spray treatment.

HiQTM Low Melting Agarose
A0223-010	GenDepot	100g	EUR 583

EDTA
20-abx082228	Abbexa	EUR 175.00 EUR 217.00	100 g 500 g

EDTA
GE4011-100G	Glentham Life Sciences	100 g	EUR 40

EDTA
GE4011-1KG	Glentham Life Sciences	1 kg	EUR 70

EDTA
GE4011-250G	Glentham Life Sciences	250 g	EUR 45

EDTA
GE4011-500G	Glentham Life Sciences	500 g	EUR 54

EDTA
B7197-1000	ApexBio	1 g	EUR 129

HyAgarose? LM Agarose, Low Melting, 100g
R9012LM-100g	ACTGene		EUR 377

HyAgarose? LM Agarose, Low Melting, 10g
R9012LM-10g	ACTGene		EUR 100

HyAgarose? LM Agarose, Low Melting, 500g
R9012LM-500g	ACTGene		EUR 1518

HyAgarose? LM Agarose, Low Melting, 50g
R9012LM-50g	ACTGene		EUR 219

EDTA plasma
90-1009	Fitzgerald	100 ml	EUR 2028
Description: BNP and NT-proBNP free plasma EDTA

EDTA Solution
2103-100	Biovision		EUR 137

Trypsin (-EDTA)
abx098244-100ml	Abbexa	100 ml	EUR 411

EDTA Na2
abx082384-500g	Abbexa	500 g	EUR 203

0.5M EDTA
E9000-010	GenDepot	100 ml	EUR 74

0.5M EDTA
E9000-050	GenDepot	500 ml	EUR 104

100G EDTA
NAT1090	National Diagnostics	100G	EUR 79

500G EDTA
NAT1092	National Diagnostics	500G	EUR 148

45UL FINE POINT TIPS FOR BECKMAN FX ROBOTICS SYSTEM,
FX-384-45FP-R	CORNING	3840/pk	EUR 2701
Description: Robotic Tips; Robotic tips - Axygen

TRYPSIN/EDTA 1X (0,25% TRYPSIN/2,21 MM EDTA)
25-053-CI	CORNING	100 mL/pk	EUR 57
Description: Media Catalog; Cell Culture Reagents

Rabbit Plasma (EDTA)
88R-1029E	Fitzgerald	500 ml	EUR 230
Description: Sterile Rabbit Plasma with EDTA

30UL FINE POINT MAXYMUM RECOVERY TIP. 384 TIPS PER RACK
VT-384-31-L-R	CORNING	3840/pk	EUR 2682
Description: Robotic Tips; Maxymum Recovery Robotic Tips - Axygen

100 ML TRYPSIN/EDTA 1X (0,05% TRYPSIN/0,53 MM EDTA)
25-052-CI	CORNING	100 mL/pk	EUR 57
Description: Media Catalog; Cell Culture Reagents

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-010	GenDepot	100ml	EUR 76

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-050	GenDepot	500ml	EUR 84

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8059-101	GenDepot	1L	EUR 126

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8150-050	GenDepot	500 ml	EUR 84

Tris EDTA Saline, 10mM Tris HCl , 1mM EDTA, 2M Nacl
T8150-801	GenDepot	8L	EUR 120

EDTA, Disodium Salt, Dihydrate
CH032	ABM	500 g	EUR 163

EDTA, Disodium Salt, Dihydrate
CH033	ABM	1.0 kg	EUR 195

DMNP-EDTA(caged calcium)
50050	Biotium	5MG	EUR 237
Description: Minimum order quantity: 1 unit of 5MG

Normal Feline Plasma (EDTA)
abx098254-50ml	Abbexa	50 ml	EUR 704

EDTA disodium salt dihydrate
GE3023-100G	Glentham Life Sciences	100 g	EUR 40

EDTA disodium salt dihydrate
GE3023-1KG	Glentham Life Sciences	1 kg	EUR 70

EDTA tetrasodium salt hydrate
GE3450-1KG	Glentham Life Sciences	1 kg	EUR 68

EDTA tetrasodium salt hydrate
GE3450-500G	Glentham Life Sciences	500 g	EUR 53

EDTA tetrasodium salt hydrate
GE3450-5KG	Glentham Life Sciences	5 kg	EUR 166

EDTA dipotassium salt dihydrate
GE5635-100G	Glentham Life Sciences	100 g	EUR 54

EDTA dipotassium salt dihydrate
GE5635-250G	Glentham Life Sciences	250 g	EUR 74

EDTA dipotassium salt dihydrate
GE5635-500G	Glentham Life Sciences	500 g	EUR 102

EDTA ferric sodium salt
GE7700-100G	Glentham Life Sciences	100 g	EUR 44

EDTA ferric sodium salt
GE7700-1KG	Glentham Life Sciences	1 kg	EUR 98

EDTA ferric sodium salt
GE7700-250G	Glentham Life Sciences	250 g	EUR 54

EDTA ferric sodium salt
GE7700-500G	Glentham Life Sciences	500 g	EUR 70

EDTA disodium salt dihydrate
GE3023-250G	Glentham Life Sciences	250 g	EUR 46

EDTA disodium salt dihydrate
GE3023-500G	Glentham Life Sciences	500 g	EUR 54

EDTA disodium salt dihydrate
GE3023-5KG	Glentham Life Sciences	5 kg	EUR 150

EDTA tetrasodium salt hydrate
GE3450-100G	Glentham Life Sciences	100 g	EUR 41

EDTA tetrasodium salt hydrate
GE3450-250G	Glentham Life Sciences	250 g	EUR 45

1L EDTA 0.5M Solution
NAT1282	National Diagnostics	1L	EUR 153

EDTA trisodium salt dihydrate
GE9701-100G	Glentham Life Sciences	100 g	EUR 54

EDTA trisodium salt dihydrate
GE9701-250G	Glentham Life Sciences	250 g	EUR 78

EDTA trisodium salt dihydrate
GE9701-500G	Glentham Life Sciences	500 g	EUR 118

EDTA tetrasodium salt dihydrate
GK0795-100G	Glentham Life Sciences	100 g	EUR 40

EDTA tetrasodium salt dihydrate
GK0795-1KG	Glentham Life Sciences	1 kg	EUR 70

EDTA tetrasodium salt dihydrate
GK0795-2500G	Glentham Life Sciences	2500 g	EUR 118

EDTA tetrasodium salt dihydrate
GK0795-250G	Glentham Life Sciences	250 g	EUR 46

EDTA tetrasodium salt dihydrate
GK0795-500G	Glentham Life Sciences	500 g	EUR 53

EDTA tripotassium salt dihydrate
GL1464-100G	Glentham Life Sciences	100 g	EUR 61

EDTA tripotassium salt dihydrate
GL1464-250G	Glentham Life Sciences	250 g	EUR 86

EDTA tripotassium salt dihydrate
GL1464-25G	Glentham Life Sciences	25 g	EUR 44

EDTA tripotassium salt dihydrate
GL1464-500G	Glentham Life Sciences	500 g	EUR 128

Trypsin-EDTA(1X), 0.25%
CA014-010	GenDepot	100ml	EUR 77

Trypsin-EDTA(1X), 0.25%
CA014-050	GenDepot	500ml	EUR 97

Trypsin-EDTA(1X), 0.25%
CA014-100	GenDepot	10x100ml	EUR 209

Trypsin-EDTA(1X), 0.25%
CA014-200	GenDepot	20x200ml	EUR 354

Trypsin-EDTA(10X), 0.5%
CA015-010	GenDepot	100ml	EUR 88

Trypsin-EDTA(10X), 0.5%
CA015-100	GenDepot	10x100ml	EUR 286

Trypsin-EDTA(10X), 0.5%
CA015-200	GenDepot	20x100ml	EUR 482

Trypsin-EDTA(1X), 0.05%
CA020-010	GenDepot	100ml	EUR 74

Trypsin-EDTA(1X), 0.025%
CA021-010	GenDepot	100ml	EUR 74

Trypsin-EDTA(1X), 0.025%
CA021-050	GenDepot	500ml	EUR 91

Trypsin-EDTA(1X), 0.025%
CA021-100	GenDepot	10x100ml	EUR 223

Trypsin-EDTA(1X), 0.025%
CA021-200	GenDepot	20x100ml	EUR 334

Trypsin-EDTA(10X), 0.05%
CA022-010	GenDepot	100ml	EUR 84

EDTA 0.5M Sterile Solution
SD8135	Bio Basic	450ml	EUR 71.75

EDTA, Ultra Pure, 1kg
R9410-1kg	ACTGene		EUR 190

EDTA, dipotassium salt, dihydrate
ED0075	Bio Basic	250g	EUR 83.06

EDTA, disodium salt, dihydrate
EB0185	Bio Basic	500g	EUR 70.01

EDTA, tetrasodium salt dihydrate
EB0436	Bio Basic	500g	EUR 88.28

30UL FINE POINT MAXYMUM RECOVERY TIP, STERILE. 384 TIPS PER RACK
VT-384-31-L-R-S	CORNING	3840/pk	EUR 2930
Description: Robotic Tips; Maxymum Recovery Robotic Tips - Axygen

15UL FILTERED FINE POINT TIP FOR V-11, CLEAR - PRE-STERILIZED
VTF-384-15UL-R-S	CORNING	3840/pk	EUR 3121
Description: Robotic Tips; Filter Tips Robotic Tips - Axygen

30UL PRE-STERILIZED FILTERED FINE POINT TIPS FOR BECKMAN FX ROBOTICS SYSTEM
FXF-384-30FP-R-S	CORNING	3840/pk	EUR 4777
Description: Robotic Tips; Filter Tips Robotic Tips - Axygen

K2-EDTA Bovine Plasma filtered
88R-1051	Fitzgerald	5 X 1 L	EUR 1650
Description: K2-EDTA Bovine Plasma filtered

Protease Inhibition Cocktail with EDTA
G135E	ABM	1.0 ml	EUR 167

Rabbit Plasma (Sterile Sodium EDTA)
20-abx800006	Abbexa	EUR 1567.00 EUR 3627.00 EUR 1149.00	100 ml 500 ml 50 ml

Rabbit Plasma (Sterile Sodium EDTA)
abx800006-1L	Abbexa	1 L	EUR 6258

Sheep Plasma (Sterile Sodium EDTA)
20-abx800023	Abbexa	EUR 662.00 EUR 829.00 EUR 565.00	100 ml 500 ml 50 ml

Sheep Plasma (Sterile Sodium EDTA)
abx800023-1L	Abbexa	1 L	EUR 996

Goat Plasma (Sterile Sodium EDTA)
20-abx800038	Abbexa	EUR 662.00 EUR 829.00 EUR 565.00	100 ml 500 ml 50 ml

Goat Plasma (Sterile Sodium EDTA)
abx800038-1L	Abbexa	1 L	EUR 996

Horse Plasma (Sterile Sodium EDTA)
20-abx800053	Abbexa	EUR 913.00 EUR 1149.00 EUR 773.00	100 ml 500 ml 50 ml

Horse Plasma (Sterile Sodium EDTA)
abx800053-1L	Abbexa	1 L	EUR 1483

Donkey Plasma (Sterile Sodium EDTA)
20-abx800068	Abbexa	EUR 1511.00 EUR 1957.00 EUR 1052.00	100 ml 500 ml 50 ml

Donkey Plasma (Sterile Sodium EDTA)
abx800068-1L	Abbexa	1 L	EUR 2555

Camel Plasma (Sterile Sodium EDTA)
20-abx800083	Abbexa	EUR 1650.00 EUR 3947.00 EUR 1288.00	100 ml 500 ml 50 ml

Camel Plasma (Sterile Sodium EDTA)
abx800083-1L	Abbexa	1 L	EUR 6759

Dog Plasma (Sterile Sodium EDTA)
20-abx800113	Abbexa	EUR 1400.00 EUR 3293.00 EUR 1080.00	100 ml 500 ml 50 ml

Dog Plasma (Sterile Sodium EDTA)
abx800113-1L	Abbexa	1 L	EUR 5687

Chicken Plasma (Sterile Sodium EDTA)
20-abx800128	Abbexa	EUR 815.00 EUR 1734.00 EUR 620.00	100 ml 500 ml 50 ml

Chicken Plasma (Sterile Sodium EDTA)
abx800128-1L	Abbexa	1 L	EUR 2973

Trypsin-EDTA (0.25%), phenol red
abx082465-100ml	Abbexa	100 ml	EUR 203

Rabbit PLASMA NS EDTA 500mL
31141-1	Pel-Freez	500mL	EUR 261.84

Rabbit PLASMA S EDTA 500mL
31156-1	Pel-Freez	500mL	EUR 285.76

CK PLASMA NS EDTA 500mL
33141-1	Pel-Freez	500mL	EUR 184.63

Bovine PLASMA NS EDTA 500mL
37141-1	Pel-Freez	500mL	EUR 184.63

Pig PLASMA NS EDTA 500mL
39541-1	Pel-Freez	500mL	EUR 184.63

EDTA magnesium disodium salt hydrate
GE3956-100G	Glentham Life Sciences	100 g	EUR 66

EDTA magnesium disodium salt hydrate
GE3956-1KG	Glentham Life Sciences	1 kg	EUR 245

EDTA magnesium disodium salt hydrate
GE3956-250G	Glentham Life Sciences	250 g	EUR 102

EDTA magnesium disodium salt hydrate
GE3956-25G	Glentham Life Sciences	25 g	EUR 45

EDTA magnesium disodium salt hydrate
GE3956-500G	Glentham Life Sciences	500 g	EUR 154

EDTA disodium zinc salt hydrate
GX3802-100G	Glentham Life Sciences	100 g	EUR 54

EDTA disodium zinc salt hydrate
GX3802-250G	Glentham Life Sciences	250 g	EUR 75

EDTA disodium zinc salt hydrate
GX3802-25G	Glentham Life Sciences	25 g	EUR 43

EDTA disodium zinc salt hydrate
GX3802-500G	Glentham Life Sciences	500 g	EUR 110

EDTA manganese disodium salt hydrate
GX6009-100G	Glentham Life Sciences	100 g	EUR 102

EDTA manganese disodium salt hydrate
GX6009-250G	Glentham Life Sciences	250 g	EUR 174

EDTA manganese disodium salt hydrate
GX6009-25G	Glentham Life Sciences	25 g	EUR 58

EDTA manganese disodium salt hydrate
GX6009-500G	Glentham Life Sciences	500 g	EUR 269

EDTA disodium and monocalcium salt
EK014P2	Bio Basic	500g	EUR 96.11

EDTA copper(II) disodium salt
EB0434	Bio Basic	250g	EUR 83.93

EDTA disodium and monomagnesium salt
EB0435	Bio Basic	500g	EUR 110.9

EDTA, Iron (III), sodium salt
EB0437	Bio Basic	100g	EUR 65.66

EDTA zinc (II), disodium salt
EB6666	Bio Basic	100g	EUR 64.79

Human Plasma Pooled Unfiltered K2-EDTA
90-1015	Fitzgerald	100 ml	EUR 336
Description: Unfiltered, pooled human plasma K2-EDTA

TAE (Tris-Acetate-EDTA) Buffer (50X)
2116-500	Biovision		EUR 120

EZBlock? Protease Inhibitor Cocktail, EDTA-Free
K272-1	Biovision		EUR 120

EZBlock? Protease Inhibitor Cocktail, EDTA-Free
K272-5	Biovision		EUR 283

EZBlock? Protease Inhibitor Cocktail, EDTA-Free
K272-set	Biovision		EUR 294

10 x TBE (Tris/Borate/EDTA)
A00269	Bio Basic	500ml	EUR 56.36

100 ML 0.5M EDTA, PH 8.0
46-034-CI	CORNING	100 mL/pk	EUR 63
Description: Media Catalog; Classical Media

EDTA buffer pH 8.0, 1000 ml
12-9160-5	Medicago	1000 ml	EUR 263

EDTA buffer pH 8.0, 500 ml
12-9161-5	Medicago	500 ml	EUR 197

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)
CE135	GeneOn	250 g	EUR 60

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)
CE136	GeneOn	500 g	EUR 72

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)
CE137	GeneOn	1 kg	EUR 104

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)
CE138	GeneOn	5 kg	EUR 349

Rabbit Plasma (Non-Sterile Sodium EDTA)
20-abx800005	Abbexa	EUR 1191.00 EUR 2806.00 EUR 746.00	100 ml 500 ml 50 ml

Rabbit Plasma (Non-Sterile Sodium EDTA)
abx800005-1L	Abbexa	1 L	EUR 4782

Rabbit Whole Blood (Sterile Sodium EDTA)
20-abx800012	Abbexa	EUR 578.00 EUR 411.00	100 ml 50 ml

Sheep Plasma (Non-Sterile Sodium EDTA)
20-abx800022	Abbexa	EUR 537.00 EUR 662.00 EUR 467.00	100 ml 500 ml 50 ml

Sheep Plasma (Non-Sterile Sodium EDTA)
abx800022-10L	Abbexa	10 L	EUR 4782

Sheep Whole Blood (Sterile Sodium EDTA)
20-abx800029	Abbexa	EUR 314.00 EUR 272.00	100 ml 50 ml

Goat Plasma (Non-Sterile Sodium EDTA)
20-abx800037	Abbexa	EUR 537.00 EUR 662.00 EUR 467.00	100 ml 500 ml 50 ml

Goat Plasma (Non-Sterile Sodium EDTA)
abx800037-1L	Abbexa	1 L	EUR 829

Goat Whole Blood (Sterile Sodium EDTA)
20-abx800044	Abbexa	EUR 314.00 EUR 272.00	100 ml 50 ml

Horse Plasma (Non-Sterile Sodium EDTA)
20-abx800052	Abbexa	EUR 718.00 EUR 913.00 EUR 634.00	100 ml 500 ml 50 ml

Horse Plasma (Non-Sterile Sodium EDTA)
abx800052-1L	Abbexa	1 L	EUR 1149

Horse Whole Blood (Sterile Sodium EDTA)
20-abx800059	Abbexa	EUR 425.00 EUR 578.00 EUR 356.00	100 ml 500 ml 50 ml

Horse Whole Blood (Sterile Sodium EDTA)
abx800059-1L	Abbexa	1 L	EUR 690

Donkey Plasma (Non-Sterile Sodium EDTA)
20-abx800067	Abbexa	EUR 1149.00 EUR 1539.00 EUR 843.00	100 ml 500 ml 50 ml

Donkey Plasma (Non-Sterile Sodium EDTA)
abx800067-1L	Abbexa	1 L	EUR 1957

Donkey Whole Blood (Sterile Sodium EDTA)
20-abx800074	Abbexa	EUR 648.00 EUR 913.00 EUR 467.00	100 ml 500 ml 50 ml

Donkey Whole Blood (Sterile Sodium EDTA)
abx800074-1L	Abbexa	1 L	EUR 1094

Camel Plasma (Non-Sterile Sodium EDTA)
20-abx800082	Abbexa	EUR 1288.00 EUR 3042.00 EUR 1024.00	100 ml 500 ml 50 ml

Camel Plasma (Non-Sterile Sodium EDTA)
abx800082-1L	Abbexa	1 L	EUR 5102

Camel Whole Blood (Sterile Sodium EDTA)
20-abx800089	Abbexa	EUR 718.00 EUR 1748.00 EUR 537.00	100 ml 500 ml 50 ml

Camel Whole Blood (Sterile Sodium EDTA)
abx800089-1L	Abbexa	1 L	EUR 2736

Using cultural, morphological, and physiological tests combined with 16S rRNA-based molecular analysis, MBFA-172 was identified as a moderately thermophilic Streptomyces thermocarboxydus-related species. Upon review, our results strongly indicated that MBFA-172 is a promising biocontrol agent for strawberry anthracnose.